KADAR FENOLIK TOTAL, FLAVONOID TOTAL, DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BINAHONG (Anredera cordifolia Ten.Steenis)

Irma Erika Herawati; Ita Inayah; Lisna Dewi

doi:10.52943/jifarmasi.v9i1.1916

Penulis

Irma Erika Herawati Sekolah Tinggi Farmasi Indonesia
Ita Inayah Universitas al-Ghifari
Lisna Dewi Universitas al-Ghifari

DOI:

https://doi.org/10.52943/jifarmasi.v9i1.1916

Kata Kunci:

phenolics, flavonoids, antioxidant, binahong

Abstrak

Antioxidant plays an important role in the body’s defense against various diseases, because antioxidant compounds were able to prevent bad effects caused by free radicals. Phenolic and flavonoids compound are known as natural antioxidant from plants. Binahong (Anredera cordifolia) is known contain phenolic and flavonoid. The aim of this research was to determine total phenolics, flavonoids content and also antioxidant activity from extract binahong using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that binahong ethanol extract had total phenolic 223.76 mg GAE/g and flavonoid content of 0.483 mg RE/g. Meanwhile, for antioxidant activity, IC50 for ethanol extract, ethyl acetate fraction, and water fraction were 717.27; 586.28; dan 425.81 ppm respectively. Binahong extracts and fractions are included in the weak antioxidant category.

Biografi Penulis

Ita Inayah, Universitas al-Ghifari

Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Lisna Dewi , Universitas al-Ghifari

Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Referensi

Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. (2019). Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants, 8(4), 96. https://doi.org/10.3390/plants8040096

Chang, C.-C., Yang, M.-H., Wen, H.-M., & Chern, J.-C. (2020). Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3). https://doi.org/10.38212/2224-6614.2748

Chun, O. K., Kim, D.-O., & Lee, C. Y. (2003). Superoxide radical scavenging activity of the major polyphenols in fresh plums. Journal of Agricultural and Food Chemistry, 51(27), 8067–8072.

Dadiono, M.S., dan Andayani, S. (2022). Potensi Tanaman Binahong Sebagai Obat Alternatif pada Bidang Akuakultur. Jurnal Perikanan Pantura (JPP), 5(1), 157-162

Fathurrahman, M. H., Herawati, I. E., Dewi, L., & Inayah, I. (2024). Total Phenolic Content, Flavonoids, And Antioxidant Activity Of Jalantir Leaves (Coryza sumatrensis). Jurnal Ilmiah Farmasi Farmasyifa, 7(1), 47–56. https://doi.org/10.29313/jiff.v7i1.3156

Fauzi, N. I., Herawati, I. E., & Hadisoebroto, G. (2023). Kadar Fenolik Total, Kadar Flavonoid, dan Aktivitas Antioksidan Dari Ekstrak Kulit Buah Nanas (Ananas comosus (L.) Merr.) Varietas Pemalang. Jurnal Mandala Pharmacon Indonesia, 9(2), 492–500. https://doi.org/10.35311/jmpi.v9i2.413

Fernandes, A. J. D., Ferreira, M. R. A., Randau, K. P., de Souza, T. P., & Soares, L. A. L. (2012). Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae). The Scientific World Journal, 2012, 1–7. https://doi.org/10.1100/2012/923462

Fidrianny, I., Wirasutisna, K. R., & Amanda, P. (2013). Senyawa antioksidan dari ekstrak etil asetat daun binahong (Anredera cordifolia (Ten.) Steenis) dari Babakan Ciparay, Bandung Selatan, Indonesia. Acta Pharm Indonesia, 38(1), 26–30.

Hamzah, H., Wirasasmita, Y., & Syafriah, W. O. (2022). The Formulation and Antioxidant Activity Test of Binahong (Anredera cordifolia) Leaves Extract Lotion. MEDULA, 9(2), 125. https://doi.org/10.46496/medula.v9i2.24014

Harborne, J. B. (1998). Phytochemical Methods (3rd ed.). Chapman and Hall.

Herawati, I. E., & Hanifah, H. N. (2018). Antioxidant activity from ethanol extract and fractions of red flame ivy (Hemigraphis colorata Hall. F.) leaf using 1, 1-diphenyl-2-picrylhydrazyl. Drug Invention Today, 10(5), 3791–3793.

Houghton, P., & Raman, A. (1998). Laboratory Handbook of the Fractination of Natural Extracts. Chapman & Hall.

Ladeska, V., Saudah, S., & Inggrid, R. (2022). Potensi Antioksidan, Kadar Fenolat dan Flavonoid Total Ranting Tetracera indica serta Uji Toksisitas terhadap sel RAW 264,7. Jurnal Sains Farmasi & Klinis, 9(2), 95. https://doi.org/10.25077/jsfk.9.2.95-104.2022

Nugraheni, T., Putri, A., Sukmawati, A., Khasanah, L., Nisa, L., Wulandari, S., Riswana, S., & Setiawan, I. (2024). Macam-Macam Metode Pengujian Aktivitas Antioksidan. Jurnal Farmasi (Journal of Pharmacy), 13(1), 39–50.

Putri, F. E., Diharmi, A., & Karnila, R. (2023). Identifikasi Senyawa Metabolit Sekunder Pada Rumput Laut Coklat (Sargassum plagyophyllum) Dengan Metode Fraksinasi. Jurnal Teknologi Dan Industri Pertanian Indonesia, 15(1), 40–46. https://doi.org/10.17969/jtipi.v15i1.23318

Rahayu, Y.E., Ismunandar, H. Mutiara, H. (2023). Potensi Daun Binahong (Anredera cordifolia (Ten) Steen.) Terhadap Penyembuhan Luka Insisi: Tinjauan Pustaka. Agromedicine, 10(1), 31-34.

Santos-Sánchez, N. F., Cañongo, R. S.-C., Villanueva, C., & Hernández-Carlos, B. (2019). Antioxidant compounds and their antioxidant mechanism. Antioxidants, 10, 1–29.

Samosir, S.R., Auliafendri, N., & Naibaho, M.I.D. (2024). Uji Efektivitas Antioksidan dari Kombinasi Ekstrak Daun Stevia (Stevia rebaundiana) dan Daun Teh Hijau (Camellia sinensis (L.) Kuntze dengan Menggunakan Metode DPPH (1,1-Difenil-2-Pikrilhidrazil). Jurnal Ilmiah Farmasi Imelda, 7(2), 105-113

Sumarina, P.O. Swastini, D.A, Ardinata, I.P.R., Suarka, I.P.S.D. (2017). Penentuan Profil Kandungan Kimia Ekstrak Etanol Daun Binahong (Anredera scandens (L.) Moq.). Jurnal Farmasi Udayana, 6(1). 23-25.

Senet, M. R. M., Raharja, I. G. M. A. P., Darma, I. K. T., Prastakarini, K. T., Dewi, N. M. A., & Parwata, I. M. O. A. (2018). Penentuan kandungan total flavonoid dan total fenol dari akar kersen (Mutingia calabura) serta aktivitasnya sebagai antioksidan. Jurnal Kimia, 12(1), 13–18.

Sholikhah, K. P., Riyanti, S., & Wahyono, W. (2023). Potensi Antioksidan Alami Rempah Bunga Honje Hutan (Etlingera hemisphaerica (Blume) R. M. Sm.) Dan Isolasi Senyawa Aktifnya. Jurnal Ilmiah Farmasi Farmasyifa, 6(2), 137–149. https://doi.org/10.29313/jiff.v6i2.11225