Pengaruh Vitamin E Terhadap Sel Darah Merah Mencit (Musmusculus,L) yang dipapari Tuak
Abstract
This research aims to knowing the effect of vitamin E for reticulocyte count, erythocytes count, and morphological description of erythrocytes, hemoglobin level and mice blood hematocrit score who given palm wine for adult category. Research using RancanganAcakLengkap (RAL), 30 male mice (L musculus) adult strain DD Webster aged 2-3 months with weight 25-30 g, who share at random into 6 trial group. The result of research show that (1) giving palm wine 0,5mg/mice/day can reduce the reticulocyte presentation if added with vitamin E 0,33 mg/mice/day for 30 days can improve back the erythrocytes count and avoid the morphology of erythrocytes fault for mice (3) giving palm wine 0,5 mg/mice/day can reduce the hemoglobin level and hematocrit score if added with vitamin E 0,33 mg/mice/day for 30 days can improve back hemoglobin and hematocrit score for mice.
References
Aitken, R.J., and Clarkson, J.S. 1988. Significance of Reactive Oxygen Species and Antioxidants in Defining the Efficacy of Sperm Preparation Techniques. Journal of Andrology, Nov/Dec; 9(6): 367-376.
Aisyah R, Shanti L, dan Tetri W., 2003. Efek pemberian Natrium Siklamat secara Oral terhadap karakteristik Hematologis tikus putih (Rattus norvegicus L.). Vol. 5, No. 2. Hal. 124-130.
Baraas F & M.Jufri (1999) Antioksidan dan Penyakit Jantung. Prima Krida Pers, Jakarta. Hal:25.
Bijanti, R, Wahjuni R.S., Utomo B., Partosoewignjo S.(1997). Penuntun Praktika Laboratorium Patologi Klinik Veteriner Bidang Studi Kesehatan Ternak Terpadu .Surabaya : Laboratorium Patologi Klinik Veteriner Fakultas Ked. Hewan Universitas Airlangga.
Defeng, (2001), Alkohol, Oxidative Stress, and Free Radical Damage
Depkes, R.I. (1992), Petunjuk Pemeriksaan Hematologi, Jakarta, Pusat Laboratorium Kesehatan.
Depkes, RI (1989), Hematologi, Jakarta, PusatPendidikanTenagaKesehatan
Evans, W. J. (2000), Vitamin E, vitamin C, and exercise. Am J Clin Nutr, 72, 647S52S.
Foster HL, Fox JG, Smell JP. 1981 The Mouse in Biomedical Research Vol I. New York: Academic Press,
Guyton, A. C.& Hall, J.E (2006), Text book of medical physiology,11 Editions
Halliwell, B. & Whiteman, M. (2004), Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol, 142, 231-55.
Hariono, B. (1998), Patologi Klinik I. Bagian Kimia Medik Veteriner, Fakultas Kedokteran Hewan, UGM Press. Yogyakarta.
Hariyatmi (2007) Efek Suplemen Antioksidan vitamin E pada kadar Lemak Peroksida darah Tikus Putih (Ratus Norvegicus L); J.MIPA 22 :1-11
H. & Traber, M. G. (2005), Vitamins E and C are safe across a broad range of intakes. Am J ClinNutr, 81, 736-45.
Hoftbrand, A.V. dan J.E. Pettit. 1987. Kapita Selekta Haematologi. Edisi 2, Penerjemah: Darmawan, I. Jakarta. EGC PenerbitBukuKedokteran.
Kusumawati, Smith & Mangkoewidjojo. (1988), Bersahabat dengan Hewan Coba,Gajah Mada University Press. Yogyakarta.
Kehrer, J.P. 1993.Free radicals as mediators of tissue injury and disease. Critical Reviews in Toxicology 23:21–48,
Knight, J.A. 1998. Free radicals: Their history and current status in aging and disease. Annals of Clinical and Laboratory Science 28:331–346, 1998.
Kusumawati, Smith & Mangkoewidjojo. (1988), Bersahabat dengan Hewan Coba Gajah Mada University Press. Yogyakarta.
Lane-Pebber W. 1976 Laboratory Mouse. The UFAW Handbook on the Care and Management of Laboratory Animal. Edinburg London New York: Churchill Livingstone,
Notoadmodjo, S. (2002), Metodologi Penelitian Kesehatan, Rineka Cipta. Jakarta.
Soewoto H., Sadikin M., Inawati S.W., Retno D., Abadi P., Dkk. (2001), Biokimia: Experimen Laboratorium, Cetakan I, WidyaMedika, Jakarta.
Slater, T. F. (1984), Free radical - mechanisms in injury, Biochem, 222, 1-15.
Smith Jhon, B.B.V.Sc. & Mangkoewidjodjo Soesano, (1988), Pemeliharaan Pembiakan dan Penggunaan Hewan Percobaan di Daerah Tropis. UI Press Jakarta.
Sugandi E, Sugiarto. (1994), Rancangan Percobaan Teori dan Aplikasi. Andi Offset Yogyakarta, 8, 24
O, H. 1993. Aren: Budidaya dan Multigunanya. Yogyakarta: Penerbit Kanisius.
Telford, R. D., Sly, G. J., Hahn, A. G., Cunningham, R. B., Bryant, C. & Smith, J. A. (2002) Footstrike is the major cause of hemolysis during running. J.Appl Physiol, 94, 38-42.
